pcr amplified dna fragment Search Results


pcr amplified dna fragments  (Nextera AS)
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Nextera AS pcr amplified dna fragments
Pcr Amplified Dna Fragments, supplied by Nextera AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr amplified dna fragments - by Bioz Stars, 2026-03
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pcr amplification of 16s rrna-encoding dna and sequencing of amplified fragments  (Janvier Labs)
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Janvier Labs pcr amplification of 16s rrna-encoding dna and sequencing of amplified fragments
Pcr Amplification Of 16s Rrna Encoding Dna And Sequencing Of Amplified Fragments, supplied by Janvier Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr amplification of 16s rrna-encoding dna and sequencing of amplified fragments/product/Janvier Labs
Average 90 stars, based on 1 article reviews
pcr amplification of 16s rrna-encoding dna and sequencing of amplified fragments - by Bioz Stars, 2026-03
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pcr amplified dna fragments purification  (Qiagen)
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Qiagen pcr amplified dna fragments purification
Pcr Amplified Dna Fragments Purification, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pcr amplified dna fragments purification - by Bioz Stars, 2026-03
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pcr amplified dna fragment  (First BASE Laboratories)
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First BASE Laboratories pcr amplified dna fragment
Pcr Amplified Dna Fragment, supplied by First BASE Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr amplified dna fragment/product/First BASE Laboratories
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pcr amplified dna fragment - by Bioz Stars, 2026-03
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pcr amplified hnst dna fragment  (Qiagen)
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pcr amplified fragments cloned into pbluescript for subsequent dna sequencing  (Eurofins)
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Eurofins pcr amplified fragments cloned into pbluescript for subsequent dna sequencing
The effect of wild type H-NST and mutant derivatives on LEE -encoded protein levels was determined by western analysis as described in <xref ref-type= Materials and Methods . EHEC strain TUV93-0 containing the empty vector pQE80 (lane 1), TUV93-0 containing constructs producing wild type EPEC H-NST (lane 2) or the H-NST mutant derivatives H-NST A16V (lane 3), H-NST A16L (lane 4), H-NST R60Q (lane 5) and HNST R60Q/R63Q (lane 6) were grown in LB to a density of OD 600 ∼0.5 and hnsT expression was induced by 0.5 mM IPTG for 60 min. Levels of EspA, EspB and GroEL were detected in total protein by western analysis using polyclonal antisera against the respective proteins as indicated. GroEL served a loading control for total protein. The relative levels of EspA and EspB normalized to that of GroEL are indicated by numbers below the protein bands. Data shown are representative of four independent experiments. " width="250" height="auto" />
Pcr Amplified Fragments Cloned Into Pbluescript For Subsequent Dna Sequencing, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr amplified fragments cloned into pbluescript for subsequent dna sequencing/product/Eurofins
Average 90 stars, based on 1 article reviews
pcr amplified fragments cloned into pbluescript for subsequent dna sequencing - by Bioz Stars, 2026-03
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pcr-amplified dna bisulfite fragments for  (Pyrosequencing Inc)
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pcr primers for amplifying dna fragments  (Metabion International AG)
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pepc - specific probe–pcr amplified pepc dna fragment (+600 to +966) labeled with horseradish peroxidase (hrp)  (Amersham Life Sciences Inc)
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Amersham Life Sciences Inc pepc - specific probe–pcr amplified pepc dna fragment (+600 to +966) labeled with horseradish peroxidase (hrp)
The effect of wild type H-NST and mutant derivatives on LEE -encoded protein levels was determined by western analysis as described in <xref ref-type= Materials and Methods . EHEC strain TUV93-0 containing the empty vector pQE80 (lane 1), TUV93-0 containing constructs producing wild type EPEC H-NST (lane 2) or the H-NST mutant derivatives H-NST A16V (lane 3), H-NST A16L (lane 4), H-NST R60Q (lane 5) and HNST R60Q/R63Q (lane 6) were grown in LB to a density of OD 600 ∼0.5 and hnsT expression was induced by 0.5 mM IPTG for 60 min. Levels of EspA, EspB and GroEL were detected in total protein by western analysis using polyclonal antisera against the respective proteins as indicated. GroEL served a loading control for total protein. The relative levels of EspA and EspB normalized to that of GroEL are indicated by numbers below the protein bands. Data shown are representative of four independent experiments. " width="250" height="auto" />
Pepc Specific Probe–Pcr Amplified Pepc Dna Fragment (+600 To +966) Labeled With Horseradish Peroxidase (Hrp), supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pepc - specific probe–pcr amplified pepc dna fragment (+600 to +966) labeled with horseradish peroxidase (hrp)/product/Amersham Life Sciences Inc
Average 90 stars, based on 1 article reviews
pepc - specific probe–pcr amplified pepc dna fragment (+600 to +966) labeled with horseradish peroxidase (hrp) - by Bioz Stars, 2026-03
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pcr amplified dna fragment  (Promega)
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pcr amplified dna fragments  (Qiagen)
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pcr-amplified 16s rrna dna fragments  (Macrogen)
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The effect of wild type H-NST and mutant derivatives on LEE -encoded protein levels was determined by western analysis as described in <xref ref-type= Materials and Methods . EHEC strain TUV93-0 containing the empty vector pQE80 (lane 1), TUV93-0 containing constructs producing wild type EPEC H-NST (lane 2) or the H-NST mutant derivatives H-NST A16V (lane 3), H-NST A16L (lane 4), H-NST R60Q (lane 5) and HNST R60Q/R63Q (lane 6) were grown in LB to a density of OD 600 ∼0.5 and hnsT expression was induced by 0.5 mM IPTG for 60 min. Levels of EspA, EspB and GroEL were detected in total protein by western analysis using polyclonal antisera against the respective proteins as indicated. GroEL served a loading control for total protein. The relative levels of EspA and EspB normalized to that of GroEL are indicated by numbers below the protein bands. Data shown are representative of four independent experiments. " width="100%" height="100%">

Journal: PLoS ONE

Article Title: H-NST Induces LEE Expression and the Formation of Attaching and Effacing Lesions in Enterohemorrhagic Escherichia coli

doi: 10.1371/journal.pone.0086618

Figure Lengend Snippet: The effect of wild type H-NST and mutant derivatives on LEE -encoded protein levels was determined by western analysis as described in Materials and Methods . EHEC strain TUV93-0 containing the empty vector pQE80 (lane 1), TUV93-0 containing constructs producing wild type EPEC H-NST (lane 2) or the H-NST mutant derivatives H-NST A16V (lane 3), H-NST A16L (lane 4), H-NST R60Q (lane 5) and HNST R60Q/R63Q (lane 6) were grown in LB to a density of OD 600 ∼0.5 and hnsT expression was induced by 0.5 mM IPTG for 60 min. Levels of EspA, EspB and GroEL were detected in total protein by western analysis using polyclonal antisera against the respective proteins as indicated. GroEL served a loading control for total protein. The relative levels of EspA and EspB normalized to that of GroEL are indicated by numbers below the protein bands. Data shown are representative of four independent experiments.

Article Snippet: Oligonucleotide sequences used for plasmid constructions are listed in . pQEH-NST: A 262 bp DNA fragment encoding hnsT was PCR amplified from EPEC E2348/69 gDNA using the primer set QEH-NST F/QEH-NSTF R, digested with Bam HI and Hind III and cloned into the corresponding sites in pQE80 (QIAGEN).

Techniques: Mutagenesis, Western Blot, Plasmid Preparation, Construct, Expressing, Control

FAS assays were used to determine the effect of H-NST on A/E lesion formation of EHEC as described in <xref ref-type= Materials and Methods . HeLa cell monolayers were co-cultured for four hours with EHEC strain TUV93-0 containing the empty vector pQE80 (A), constructs producing wild type EPEC H-NST (B) or the H-NST mutant derivatives H-NST A16V (C), H-NST A16L (D), H-NST R60Q (E) and HNST R60Q/R63Q (F). The images of FITC phalloidin-stained actin of infected HeLa cells are representative of three independent experiments. Arrows indicate examples of A/E lesions. " width="100%" height="100%">

Journal: PLoS ONE

Article Title: H-NST Induces LEE Expression and the Formation of Attaching and Effacing Lesions in Enterohemorrhagic Escherichia coli

doi: 10.1371/journal.pone.0086618

Figure Lengend Snippet: FAS assays were used to determine the effect of H-NST on A/E lesion formation of EHEC as described in Materials and Methods . HeLa cell monolayers were co-cultured for four hours with EHEC strain TUV93-0 containing the empty vector pQE80 (A), constructs producing wild type EPEC H-NST (B) or the H-NST mutant derivatives H-NST A16V (C), H-NST A16L (D), H-NST R60Q (E) and HNST R60Q/R63Q (F). The images of FITC phalloidin-stained actin of infected HeLa cells are representative of three independent experiments. Arrows indicate examples of A/E lesions.

Article Snippet: Oligonucleotide sequences used for plasmid constructions are listed in . pQEH-NST: A 262 bp DNA fragment encoding hnsT was PCR amplified from EPEC E2348/69 gDNA using the primer set QEH-NST F/QEH-NSTF R, digested with Bam HI and Hind III and cloned into the corresponding sites in pQE80 (QIAGEN).

Techniques: Cell Culture, Plasmid Preparation, Construct, Mutagenesis, Staining, Infection